GEMBAS
The main research themes of the GEMBAS Team extend to the understanding of the understanding and use of the mechanisms of interactions between biomolecules or cellular components with electrochemical, optical and/or 3D materials while keeping the understanding of biochemical phenomena as a framework.
Our team
The scientific activity of this group is built around the analysis of molecule-membrane interactions using both biomimetic (Langmuir monolayers, tethered lipid bilayers) and « natural » cell membranes. We design membrane biomimetic models for molecule-membrane analysis, and lipid carriers for drug delivery and investigations of their interactions with cell plasma membranes.*
The group focuses on bioelectrochemical and electrochemiluminescent interfaces (e.g. immobilisation of biological components on electrode surfaces leading to electrochemical and/or chemiluminescent signals) for applications in health (enzyme inhibitor detection), environment (pesticide detection) and transformation of lignocellulosic biomass. Scientific expertises and specialities are enzymology, molecular biology, electrochemical screening and electrochemiluminescence.*
The research theme we are currently developing is mainly focused on understanding the molecular and interfacial mechanisms of enzymatic lipolysis, especially lipases and phospholipases. Understanding the specificity of action of (phospho)lipases, and being able to modify it, is an important issue because these lipolytic enzymes have many fields of biotechnological and medical applications.*
The research activities of the group focus on the study of the interfaces between materials, process and biological components. The group research projects are spread in the following fields:
- Biomolecule / polymer interface: surface chemistry, microarrays and sensing surfaces developments
- Cell / biomaterial interaction: hydrogels formulation, 3D bioprinting concept developments, tissue engineering, regenerative medicine
- Process engineering: Robotic and Freeform 3D printing (FRESH, POS (Dynamic Molding)) applied to additive manufacturing and bioprinting
- Artificial intelligence / numerical simulation applied to additive manufacturing and 3D bioprinting
- Bioprocesses: concepts development for tissue engineering and 3D bioproduction
- Additive manufacturing of medical devices (ISO 13485:2016 certification)
The group is coordinating the 3d.FAB Technological Innovation Platform.
The group is hosting a common laboratory with SARTORIUS, the 3DInnovationLab.
Publications Find the most recent scientific contributions
Herbicide detection: A review of enzyme- and cell-based biosensors
Octobre G., Delprat N., Doumèche B., Leca-Bouvier B.
Environ. Res. 2024, 249:118330
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Structural determination and kinetic analysis of the Transketolase from Vibrio vulnificus reveal unexpected cooperative behavior.
Georges, R.-N., Ballut, L., Octobre, B., Comte, A., Hecquet, L., Charmantray, F.*, Doumèche, B.
Protein Science, 2024, 33(3):e4884
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Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities
Fall, I., Doumèche, B., Abdellaoui, S., Rémond C., Rakotoarivonina, H., Ochs M.
Bioelectrochem. 2023, 156, 108609
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User-friendly one-step disposable signal-on bioassay for glyphosate detection in water samples
Delprat, N., Martins, L.O., Blum, L.J., Aymard, C.M.G., Leca-Bouvier, B., Octobre, G., Doumèche, B.
Biosens. Bioelecton. 2023, 241, 115689
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Round-robin testing of commercial two-photon polymerization 3D printers.
Cantoni F., Maher D., Bosler E., Kühne S., Barbe L., Oberschmidt D., Marquette C., Taboryski R., Tenje M., Bunea, A.-I